The Effects of Oxaliplatin Genotoxicity on Human Lymphocytes Free Essay Example, 3750 words

It has been amply indicated that the primary mechanism of cell death induced by platinum compounds is through the formation of DNA adducts (Almeida et a. l, 2005; Hah et al. , 2007; Boysen et al. , 2009). These DNA adducts are comprised of intrastrand and interstrand crosslinks that result from covalent bonding between the platinum atom and DNA bases (Hah et al. , 2007). The ability of a modified alkaline Comet assay was investigated to measure the crosslinks that are induced by oxaliplatin. For this, 50 M H2O2 was used with the consideration that it would strongly induce strand and double strand breaks, and the reduced detection of these breaks would serve to identify the action of a cross-linking agent. It was demonstrated that DNA breaks were reduced in presence of cross-links. REF Hence, lymphocytes were pre-treated in vitro with different concentrations of oxaliplatin to induce cross-links in order to decrease the H2O2-induced DNA migration with the Comet assay. Our data showe d that an inverse relation exists between the increased concentration of oxaliplatin-induced DNA crosslinks and H2O2-induced DNA migration as measured by decreased OTM (Figure 1). We will write a custom essay sample on The Effects of Oxaliplatin Genotoxicity on Human Lymphocytes or any topic specifically for you Only $17.96 $11.86/page Specifically, oxaliplatin was shown to form 1,2-GG intrastrand DNA crosslinks (Malina et al. , 2007).